dCas9-KRAB mRNA (m1ψ substitution)
dCas9-KRAB mRNA encodes the dCas9-KRAB fusion protein, a tool for gene silencing and transcriptional repression. The dCas9 (dead Cas9) component targets DNA precisely without cutting, while the KRAB (Krüppel-associated box) domain recruits complexes to inhibit gene expression. Delivered as mRNA, dCas9-KRAB ensures rapid, high transient expression without nuclear uptake, making it ideal for research in gene function, genetic diseases, and therapeutic development.
Croyez’s dCas9-KRAB mRNA is produced through in vitro transcription, featuring a Cap1-modified 5' end, a poly(A) tail at the 3' end, and modified nucleotides to reduce immune response. These enhancements ensure stability and performance, allowing the mRNA to function like natural mature mRNAs in cells.
Croyez’s dCas9-KRAB mRNA is produced through in vitro transcription, featuring a Cap1-modified 5' end, a poly(A) tail at the 3' end, and modified nucleotides to reduce immune response. These enhancements ensure stability and performance, allowing the mRNA to function like natural mature mRNAs in cells.
mRNA Length:
4982 nt
Base Composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, gene regulation studies, therapeutic development, epigenetic research.
Shipping Conditions:
Dry ice
4982 nt
Base Composition:
N1-Me-pUTP (N1-mψ)
Concentration:
1.0 mg/ mL
Cap Modification:
Cap 1 structure
Poly A Tail:
Yes
Form:
Liquid
Buffer:
1 mM sodium citrate buffer, pH 6.4.
Storage:
Products can be stored at -80°C or below.
We recommend to aliquot the mRNA solution for a better storage. Avoid repeated freeze/thaw cycles.
Shipping:
The products are shipped on dry ice and should be avoided for freeze-thaw cycles.
Application:
Gene function research, gene regulation studies, therapeutic development, epigenetic research.
Shipping Conditions:
Dry ice
